http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2913686-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_42a59c5faed1d0699d86edc5a2eee671 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6441 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-952 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5014 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5058 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5073 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-94 |
| filingDate | 2014-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_29697c133306425bc8c925a60373c001 |
| publicationDate | 2014-12-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2913686-A1 |
| titleOfInvention | Means and methods for the determination of the biological activity of neurotoxin polypeptides in cells |
| abstract | The present invention pertains to methods and kits for determining the biological activity of Clostridial Neurotoxins. The method of the invention allows for the direct determination of the biological activity of a Neurotoxin polypeptide in cells. Cells susceptible to a Neurotoxin, e.g. neurological cells, are incubated with the neurotoxin (e.g. BoTN/ A), then fixed, and stained using an antibody specifically binding the cleaved neurotoxin (e.g. SNAP-25) and an antibody binding to both cleaved and uncleaved neurotoxin, for determination of total amount or content of neurotoxin substrate in the cells. The biological activity of said neurotoxin polypeptide is determined directly in the cells, and calculated by means of detection of the two complexes. |
| priorityDate | 2013-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 390.