| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e138e116e360a82206cbc5716cc95919 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-66
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2014-03-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9721cee111288ee3d2612266808310cc
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e7af6a4697330cc21e83ba1fd678102f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8dda1453b56770b993986af16f39722d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fa2b1076c3e05b8786ab131f83f569cf
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3840f0b517b8e4a30a012944e35c31ad |
| publicationDate |
2014-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CA-2907146-A1 |
| titleOfInvention |
Chemically modified ligase cofactors, donors and acceptors |
| abstract |
Provided herein are methods for ligation of polynucleotides containing modified ligation components, particularly modified ligase cofactors, modified acceptors and modified donors. The methods readily applied to ligation-based assays for detection of a nucleic acid sequence where the use of the modified cofactor improves discrimination between matched and mismatched templates. Furthermore, the use of the modified ligation components reduces or eliminates the ligation in the absence of nucleic acid template. In addition, methods are applied to the preparation of nucleic acid libraries using modified acceptor probes and modified donor probes that reduce or eliminate probe dimerization during the ligation process. |
| priorityDate |
2013-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |