| abstract |
The invention relates to a process for the production on an industrial scale of capsular polysaccharide of Haemophilus influenzae type b (PRP) for vaccine purposes, according to which a strain of Haemophilus influenzae type b (Hib) is cultured in a culture medium, the culture supernatant is collected which is treated to extract the capsular polysaccharide, said culture medium comprising at least: a carbon source, protoporphyrin, salts, amino acids, NAD or NADH, vitamins, means for regulating pH, characterized in that said culture medium is chemically defined. |