Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bda5416e591d3d33059f20c4f65f9c90 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2320-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-80 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-531 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-3513 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6811 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-902 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 |
filingDate |
2013-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7cb95386c6b1f48fd363e275d02760e4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d0f74949e5557cbd070843eff270882 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b046750b543b8c1a18618bf313f026b2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db2b2a237bcb4e74ddd8733f5f240b88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ab62a8ee9c475fe72a9aa8f5bc6d49af http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0029ce6b865e8533ad4d18f4ba9246b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a45543f9397c3530eea306bb96e716fa |
publicationDate |
2013-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CA-2867849-A1 |
titleOfInvention |
Rna-directed dna cleavage by the cas9-crrna complex |
abstract |
Isolation or in vitro assembly of the Cas9-crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule in vitro or in vivo using an RNA-guided DNA endonuclease comprising RNA sequences and at least one of an RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one point mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro and using a cassette containing a single repeat-spacer-repeat unit. |
priorityDate |
2012-03-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |