| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_567bfd99de4692908499990940803693 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-689 |
| filingDate |
2014-08-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2022-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0bc752b4d58671b60da49c3404ca03c3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db58498441a3f862f65f4053f4e6e284
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5363ff68628dcc3c182196ddf3b6defa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_10019d2196c71d5dc7370dd8b9be65a3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ed00c286db65734909d0a481f2d3a65
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52e81dfa6649d51aca253dab553ce0af
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4d6bb1fabbb9221bcf645c7a8d5f2888 |
| publicationDate |
2022-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CA-2859890-C |
| titleOfInvention |
Method for determining the presence or absence of shiga toxin-producing escherichia coli (stec) in a food sample |
| abstract |
A method shortens period of time to determine the presence or absence of pathogenic STEC (Shiga toxin-producing Escherichia coli). Food samples are incubated in a culture medium to obtain an E. coli stock medium. The E. coli is lysed and the sample is subjected to a first series of polymerase chain reactions using primers to amplify the following E. coli genes or fragments: stxl and/or stx2; for each STEC serogroup to be determined, the subtype of the eae gene of that serogroup encoding an intimin, and for each STEC serogroup to be determined, a biomarker gene specific for that serogroup. The presence of the one or more specific serogroups of STEC is verified by performing a second series of PCRs on concentrates of the E. coli stock medium and, optionally, a third series of PCRs on single E. coli colonies derived from the concentrates. |
| priorityDate |
2013-09-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |