| abstract |
The present invention provides a method for selecting proteins with one or more enhanced phenotypic properties of surface expression level, stability, secretion levels and solubility relative to those of a wild-type of said protein. Yeast cells are transformed with a vector expressing a protein to be tested fused to a yeast cell wall protein. Mutagenesis is used to a generate a variegated population of mutants of the protein to be tested. The yeast cells are labeled with a first label which associates with yeast expressing the protein having one or more enhanced phenotypic properties of surface expression level, stability, secretion levels and solubility and does not associate with yeast which do not express the protein having enhanced phenotypic properties, and the transformed yeast cells with which the first label is associated are isolated. After analyzing and comparing the phenotypic properties of the mutant protein expressed by yeast with phenotypic properties of the wild-type protein, yeast cells exhibiting proteins with one or more enhanced phenotypic properties of surface expression level, stability, secretion levels and solubility over the wild-type protein are selected. |