| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ca45d719b6774f7cbaf1ddd7ac03a5de |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-1077 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-1077
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-026
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-5437 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07C279-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
| filingDate |
2012-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_289b136c94301bdb0d589e6091794771
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eec026881abdfcc7fc8eecec3e6cbd8d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c9c5d115f09bde5b0c05b9be04cbe687
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8fe3c100810bf520c3fe4a8036c3600f |
| publicationDate |
2013-04-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CA-2849752-A1 |
| titleOfInvention |
Method for producing polypeptide fragment with high efficiency, which is suitable for ncl method |
| abstract |
[Problem] To provide a method for producing a polypeptide fragment with high efficiency, which is suitable for an NCL method. [Solution] A production method comprising: a step of reacting a polypeptide which comprises a first polypeptide fragment having a cysteine residue as an N-terminal thereof and a second polypeptide fragment with CNBr to produce the first polypeptide fragment having a cysteine residue as an N-terminal thereof and a third polypeptide fragment, wherein the first polypeptide fragment and the second polypeptide fragment are linked to each other through an intervening sequence -Cys-W-(His)n-Z-Met-; and a step of reacting the third polypeptide fragment with a compound represented by formula (I) and a compound represented by formula (II) sequentially to produce the second polypeptide fragment having a modified C-terminal. |
| priorityDate |
2011-09-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |