http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2828138-C
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_382ccfc2ebebcaee15aa7cda4cee86bc |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-734 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0663 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-075 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-24 |
| filingDate | 2013-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_949b8c4f6904ec84b83bec0553be70eb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_12e5588b12bc6bed2bd016d2261dd651 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_70f579d43912a00c38f3534d2cfb7773 |
| publicationDate | 2015-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2828138-C |
| titleOfInvention | Method of washing adherent cell using trehalose-containing cell-washing solution |
| abstract | It is to provide a method of washing an adherent cell, capable of effectively suppressing cell death due to proteolytic enzyme treatment for detaching the adherent cell from a culture vessel and subsequent cell treatment; a cell-washing solution used for the washing method; a method of producing a cell suspension for transplantation using the cell-washing solution; and a kit comprising the cell-washing solution. Trehalose or its derivative or a salt thereof is added to a physiological aqueous solution to prepare a cell-washing solution containing trehalose or its derivative or a salt thereof as an active ingredient. The cell-washing solution can be used to wash an adherent cell before detaching the adherent cell from a culture vessel by proteolytic enzyme treatment to suppress (inhibit) cell death (apoptosis or the like) due to the proteolytic enzyme treatment. The concentration of trehalose applied to the cell-washing solution may be a concentration capable of suppressing (inhibiting) the cell death (apoptosis or the like) due to the proteolytic enzyme treatment, and is typically 0.1 to 20 (w/v)%, preferably 1 to 15 (w/v)%, more preferably 1.0 to 7.0 (w/v)%, still more preferably 3 to 5.0 (w/v)%. |
| priorityDate | 2012-09-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 365.