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filingDate 2009-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4107be2e72ba18921ec004f03d185f7e
publicationDate 2010-06-03-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CA-2779197-A1
titleOfInvention Method of removing polymerase chain reaction (pcr) inhibitors from a sample using .beta.-cyclodextrin and activated charcoal coated with bentonite
abstract Abstract The present invention provides formulations and methods for isolating food-borne pathogens using bentonite-coated activated charcoal from samples, including a great variety of food matrices. Methods for isolating microorganisms from clinical and environmental specimens are also disclosed. The invention also concerns methods for rapid and efficient isolation of sufficiently pure DNA from small amounts of various pathogenic microorganisms and samples using bentonite-coated activated charcoal through simple binding. The isolated pure DNA can then be used, according to the methods of the instant invention, for selective identification using polymerase chain reaction (PCR) of a live pathogenic microorganism present in a sample from which the microorganism was isolated. The methods of the instant invention are also useful for identification of new pathogenic microorganisms, diagnostics of food-bome illnesses, treatment of food-borne diseases and quality control of food items offered for sale to consumers.
priorityDate 2008-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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