| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a07725ba314fda488fd0128563842e38 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H19-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H19-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09B15-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09B57-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09B69-109
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09B11-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C09B19-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-42
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 |
| filingDate |
2002-08-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2018-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a4ae649711f614b40e427445e7c2123b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b07da96eec6b10cc40b9a948c294f6c8
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_370ff45260f57b27c98d6579e2ca8563
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9045f21de43529cecb05c1a6ceced8ae |
| publicationDate |
2018-10-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CA-2774344-C |
| titleOfInvention |
Terminal-phosphate-labeled nucleotides and methods of use |
| abstract |
The present invention describes methods of detecting a nucleic acid in a sample and methods of determining the identity of a single nucleotide in a nucleic acid sequence, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real- time monitoring of DNA or RNA synthesis and detection of a target nucleic acid. |
| priorityDate |
2001-08-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |