http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2747479-C
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c1432597c4e83d4ec79685539afb3f1d |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B30-04 |
| filingDate | 2009-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2017-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5de8864a7a28966eca965cbb80333358 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0b16d6a9d864bca82e39af35e5d52b76 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea94dd84fe5f3db8565efd33a06efa07 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_487c6f2b291dddf10a5139ea47eac306 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c82f84718d49b875fb08bb97ad2be4a6 |
| publicationDate | 2017-11-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2747479-C |
| titleOfInvention | Methods and reagents for shortening incubation times in hybridization assays |
| abstract | The methods and reagents described herein can be used to shorten incubation times in hybridization assays. As demonstrated in the examples, we have identified specific sulfonic acid polymers and hybridization conditions that lead to significantly shorter incubation times (e.g., signals after three hours that are comparable to signals that could traditionally only be obtained after overnight incubation). In some embodiments, shorter incubation times are achieved by adding the sulfonic acid polymer(s) during the hybridization process. Alternatively or additionally, in some embodiments, shorter incubation times are achieved via changes to the hybridizing conditions, e.g., by reducing the hybridization volume, increasing the salt concentration, and/or increasing the probe concentration (capture extender probe and/or label extender probe). |
| priorityDate | 2008-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 75.