http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2747479-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efd09c296645384c4e1c770411f7aa01 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6834 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B30-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2009-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c892405183a593286a4e4372ac9ba3c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f82700f7ee8bb1b49fcf7b1133c27fbc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0145f7e4b3074328c561138296254169 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ce6b75d2960e919f9723769899a6e17e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_46625887a992bf7a36437571219bee7b |
| publicationDate | 2010-07-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2747479-A1 |
| titleOfInvention | Methods and reagents for shortening incubation times in hybridization assays |
| abstract | The methods and reagents described herein can be used to shorten incubation times in hybridization assays. As demonstrated in the examples, we have identified specific sulfonic acid polymers and hybridization conditions that lead to significantly shorter incubation times (e.g., signals after three hours that are comparable to signals that could traditionally only be obtained after overnight incubation). In some embodiments, shorter incubation times are achieved by adding the sulfonic acid polymer(s) during the hybridization process. Alternatively or additionally, in some embodiments, shorter incubation times are achieved via changes to the hybridizing conditions, e.g., by reducing the hybridization volume, increasing the salt concentration, and/or increasing the probe concentration (capture extender probe and/or label extender probe). |
| priorityDate | 2008-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 80.