Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_da26c5b8806cc8862294b8a59ab3098e |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-107 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8509 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-64 |
filingDate |
2009-07-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8b1d03f4b387eb026f420112fcf3e116 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_525aa0f9c15bc867e68455438d24f9c9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c3d2c3e76f88043f365b69bb7494de4c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_72ee8bd678ed8fcc550cf2042329501f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1f3e2e9bc59882e407f10fb372bccec7 |
publicationDate |
2010-01-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CA-2733119-A1 |
titleOfInvention |
Recombinant expression vector for animal cell |
abstract |
The present invention relates to a recombinant expression vector for an animal cell containing a dihydrofolate reductase (DHFR) coding nucleotide sequence operatively linked to-a DHFR promoter, to an animal cell line transformed by the vector, and to a method for preparing a target protein using the same. As compared with existing animal cell expression vectors, the vector of the present invention enables an effective screening of a cell line done in which foreign genes are amplified together with DHFR genes even at a much lower methotrexate concentration. The present invention exhibits excellent effects in cell line preparation as high-productivity cell lines can be ensured in a short time through the use of a lower concentration of methotrexate in the process of protein production cell line establishment |
priorityDate |
2008-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |