| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a1807d6c95be41785a3acbf1de3dbdfe
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_78587acbfc8d4c9e9ac3baa7021087d7 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B60-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00376
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00605
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00608
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00612
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00637
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00626
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00653
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00677
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00659
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00691
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00722
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00387
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00497
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-005
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00529
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J2219-00576 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J19-0046 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B60-14 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B60-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B05D3-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B30-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6837
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N37-00 |
| filingDate |
2002-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2012-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7e9d68568768f0f9dcf03b6b42ce9529
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f9892974f3a8a35d64d37f298ac299ba
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c1865fead9cfde156fea10eb14525b39
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1580d2bbd4936f3c78d1f0916b7b7712
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7b6faa22f6f1e7c5d196b649f5aa77dd |
| publicationDate |
2012-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CA-2656209-C |
| titleOfInvention |
Methods and devices based upon a novel form of nucleic acid duplex on a surface |
| abstract |
The present invention relates to simple method to fabricate DNA hybridization devices based upon adsorptive attachment~ of oligonucleotides to a positively charged surface. Such adsorbed oligonucleotide probes form a densely packed monolayer, which retains capacity for base-pair specific hybridization with a solution state nucleic acid target strand to form the duplex. However, both strand dissociation kinetics and the rate of DNase digestion suggest on symmetry grounds that solution-state nucleic acid binds to such adsorbed oligonucleotides to form a highly asymmetric and unwound duplex, with structural details that are substantially different from that known for the Watson-Crick DNA duplex. This novel nucleic acid duplex form can serve as the basis for a new class of hybridization device and methods for their use. It is also disclosed that new methods of nucleic acid duplex detection can be developed which are based upon the interaction of enzymes and dye labels with the unique structural characteristics of the non-helical duplex described herein. Preferred implementations of the invention include DNA microarrays, bead- based nucleic acid analysis, microelectronic devices to detect nucleic acid hybridization and more traditional methods of laboratory analysis, including hybridization on membranous and other solid supports. |
| priorityDate |
2001-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |