Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_92d71a3e23389a3bca747290fc6464cf http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7ae739b067fda3a4d47f77aa1dc7f66c |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-99 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2506-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-115 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-235 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-90 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-25 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-02 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0657 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0602 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-04 |
filingDate |
2007-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2013-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e75287bf85a1e86aff3e4c1abff71aea http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61c73095d8d5c51d9172ed3c5ab1a62c |
publicationDate |
2013-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CA-2640644-C |
titleOfInvention |
A method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetuses |
abstract |
An object of the present invention is to develop a method for purify cardiomyocytes at a high degree of purification and at a high yield from a cell mixture comprising cardiomyocytes derived from fetuses and stem cells using various features which have not been previously expected to be used for purification of cardiomyocytes or which are newly found, wherein said method is carried out without undergoing any genetic modification or without adding any special proteins or biologically active agents. The inventors of the present invention found that cardiomyocytes were effectively and highly selected and purified by culturing cardiomyocytes derived from embryonic stem cells in the culture medium under a condition selected from a low-serum-supplemented condition, a low-glucose-supplemented condition, a low-nutritional condition, a low calcium condition, a mildly-acidic pH condition, a lactic acid-supplemented condition, an aspartic acid/glutamic acid-supplemented condition, and/or a pyruvic acid-supplemented condition. The inventors of the present invention further found that the above method invented in relation to embryonic stem cells was applicable to select and purify cardiomyocytes derived from fetuses or adult stem cells. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9018010-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9404079-B2 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9040297-B2 |
priorityDate |
2006-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |