| abstract |
It is intended to provide a method, upon introducing an extracellular substance such as a sperm, a cell nucleus, a nucleic acid or a protein into a mammalian ovum, for introducing the extracellular substance into the mammalian ovum, which is safe for the mammalian ovum, enables efficient introduction, and is technically easy. By treating the mammalian ovum with a tubulin polymerization inhibitor such as vinblastine, membrane fusion between the ovum and the sperm is promoted and without making a hole in the cell membrane, the sperm and the ovum are fused and fertilization can be achieved. The method of the invention can be applied not only to the case where a sperm is introduced into a mammalian ovum, but also to a case where an extracellular substance such as a cell nucleus, a nucleic acid or a protein is introduced into a mammalian ovum. |