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filingDate 2005-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ace568e9202af8ed99386b5eb21ec0ea
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8cf33ef2489ce0bde58c371293a4b976
publicationDate 2006-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CA-2573591-A1
titleOfInvention Method of in-vitro detection and quantification of hiv dna by quantitative pcr
abstract Process for the detection and quantification in-vitro of HIV DNA by quantitative PCR. The invention consists of developing pairs of oligonucleotides capable of hybridizing with fragments of the gag gene sequence present in the genome of the HIV virus. These oligonucleotides permit amplification of the viral DNA by quantitative PCR. Even in the case of samples with low viral load, the invention permits detection of the HIV DNA virus by juxtaposition either of a conventional PCR with a quantitative PCR, or with a double nested quantitative PCR, using different pairs of primers in each amplification. The invention, compared with the known methods, allows determining qualitatively and quantitatively, in-vitro, the presence of HIV DNA in samples, in a rapid, reproducible manner and with high sensitivity.
priorityDate 2004-08-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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