| abstract |
The present invention is directed to lipid-based formulations for delivering, e.g., introducing, nucleic acid-lipid particles to a cell, and assays for optimizing the delivery efficiency of such lipid-based formulations. The nucleic acid-lipid particles comprise an interference RNA molecule, a cationic lipid with alkyl side chains from about 10 to about 20 carbon atoms having more than a single site of unsaturation, a non~cationic lipid and a conjugated lipid that inhibits aggregation of the particle such as a polyethyleneglycol (PEG)-lipid conjugate or a polyamide (ATTA)-conjugate). |