| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d202e9e866984fe229f6facc38f1d740 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-69
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
| filingDate |
2005-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc02993d67aa7cbb0fce288e56e14ffa
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_af6022992175d57c7be3c7e6f40d1e6a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_003b2546cf3757866d1c0973a42473f6
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d85dc2d8e9b2129658bb301964c6a43
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e96aa5dd63042d2e7f20ac41e64bf05a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_00aa5613a08f806b82a855f3c2e5135d |
| publicationDate |
2005-08-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CA-2554534-A1 |
| titleOfInvention |
Process for large scale production of plasmid dna by e. coli fermentation |
| abstract |
The present invention relates generally to a method for increasing the yield of plasmid DNA production. The method includes the steps of selecting a high ly productive clonal subtype of a strain of E. coli, including but not limited to the DH5 strain, harboring a DNA plasmid and cultivating said clonal subtype with fed-batch fermentation in a chemically-defined medium. The plasmid DNA production process described herein can generate record quantities of plasmi d DNA when said highly productive clonal subtypes are cultivated on an industrial scale. The disclosed method can be used for the production of pharmaceutical grade DNA for use in polynucleotide vaccination and gene therapy treatment regimens. |
| priorityDate |
2004-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |