http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2527216-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ec5b8c0fc1f827c1360a8f5ea5652225 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_efda7341819f1a85d7f2fe104cd8a26f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2004-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f2c3bba48e7a0ba7230422f02265aaf4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_765e7f861f676c897a8c1987fdde6f67 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_55824a79c11af94b1413ad8dbff5b627 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2caad1916f55b727f9994f86d37f7f03 |
| publicationDate | 2004-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2527216-A1 |
| titleOfInvention | Process for obtaining plasmid dna by means of an aqueous 2-phase system |
| abstract | The invention relates to a method for the isolation of plasmid-DNA from biomasses, by means of an aqueous biphasic system, comprising a polymer and a salt component, characterised in that the resuspension of the applied biomass, the alkaline lysates of the biomass, the neutralisation of the alkaline lysate component and the separation of the plasmid-DNA from the contaminants (such as cell remains, RNA and gDNA, for example) is carried out in a single reaction vessel (one-pot method). According to the invention, the above is achieved, whereby the neutralisation of the alkaline lysate component is carried out in one and the same container by the addition of potassium phosphate and hence one component of the aqueous biphasic system is already present and the second component of the aqueous biphasic system is a PEG with a molecular weight of calculated average from about 600 g/mol to 1000 g/mol, however, said second component is preferably formed by a mixture of PEG 600 and PEG 1000. |
| priorityDate | 2003-05-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.