http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2518452-A1
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3d929371b47ae471de44e2dbe929c73e |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q- |
filingDate | 2004-03-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_28c30d3e224e0b7318b28d9304722126 |
publicationDate | 2004-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CA-2518452-A1 |
titleOfInvention | Reca-assisted allele specific oligonucleotide extension method for detecting mutations, snps and specific sequences |
abstract | A method for detecting a specific sequence, a mutation and/or a polymorphisms, including a SNP, is based on the use RecA or a RecA-like recombinase protein and the process of allele specific oligonucleotide extension. RecA coated, specific DNA oligonucleotide probes (RecA filaments) are used for homology searching in duplex DNA. Location of homologous sequences results in the formation of D-loop or double D-loop structures containing a duplex regions comprising the oligonucleotide probe and one strand of the target DNA. Probes are selected to terminate with their 3' end at the site of the mutation or the SNP, such that extension depends on correct nucleotide pairing, which occurs only when the probe is annealed to a target DNA which comprises the allele complementary to the 3' end of the probe. Successful extension is diagnostic of the specific sequence, mutation or SNP. Also provided are compositions and kits useful for practicing the above methods. |
priorityDate | 2003-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 100.