http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2505871-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_78e5932bb36b970f92028787fe81b4a6 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2545-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2547-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2561-113 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2547-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-113 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6841 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2003-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8fd5753b2fb2d793e3be50ff40de8edf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1a3865b084cdd06036b4b532355866f8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6752eb9cbb43026c2db40756fac7714b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b53dc383ed75bed0abe6a91889c5bc43 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f987778057a9d9d9538015952bd8e991 |
| publicationDate | 2004-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2505871-A1 |
| titleOfInvention | Methods and compositions for detecting telomerase activity |
| abstract | A method for determining telomerase activity using primer extension followed with real time PCR quantification is disclosed. The method of the present invention provides a rapid, sensitive and accurate measurement for telomerase activity in a biological sample. In one embodiment, the method includes the steps of: adding the biological sample to a reaction tube containing a first reaction mixture having a first primer and nucleoside triphosphates, a second reaction mixture having a second primer and a DNA polymerase, and a wax layer that separates the first reaction mixture from the second reaction mixture; incubating the biological sample with the first reaction mixture; admixing the extension product with the second reaction mixture; amplifying and quantifying the extension product using real-time PCR and a control template. In another embodiment, the detection method includes an in situ primer extension step that allows the production of the extension product within an intact cell. In this embodiment, the extension product can be preserved under appropriate conditions for an extended time before the completion of the quantification step. |
| priorityDate | 2002-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 136.