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filingDate 2003-07-17-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2015-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_399c75cb1c2edb823a9b3c193509ce68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_17da00f9c854e530357dd2412289c932
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publicationDate 2015-06-02-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CA-2492966-C
titleOfInvention Method for the expression of unknown environmental dna into adapted host cells
abstract The present invention relates to methods for the identification or cloning of polynucleotides encoding a selected phenotype, particularly from environmental DNA. In a specific embodiment, the method comprises (i) cloning environmental DNA fragments into Ecoli cloning vectors to produce a metagenomic library, (ii) identifying or selecting cloning vectors in said library which contain DNA fragments having a particular characteristic of interest, (iii) modifying the identified or selected cloning vectors into shuttle or expression vectors for transfer and integration in a selected host cell, (iv) transferring the modified cloning vectors into said selected host cell and (v) identifying or cloning the DNA fragments contained in said modified cloning vectors which encode said selected phenotype in said selected host cell.
priorityDate 2002-07-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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