http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2492324-A1
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e5f2d89a8c9d985b85a5d6512df76351 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B30-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6842 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6845 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K17-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K17-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B30-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
filingDate | 2002-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf9d9cb4bef7e7efa09c48a786b9e981 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3e78b179e024747a67ea5b8356778e26 |
publicationDate | 2004-03-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CA-2492324-A1 |
titleOfInvention | Method for isolating subpopulations of proteins that engage in protein-protein interactions |
abstract | The invention provides a method for isolating and identifying proteins participating in protein-protein interactions in a complex mixture. The meth od uses a chemically reactive supporting matrix to isolate proteins that in tur n non-covalently bind other proteins. The supporting matrix is isolated, and t he non-covalently bound proteins are subsequently released for analysis. Becaus e the proteins are accessible to chemical manipulation at both the binding and release steps, identification of the non-covalently bound proteins yields information on specific classes of interacting proteins, such as calcium- dependent or substrate-dependent protein interactions. This permits selectio n of a subpopulation of proteins from a complex mixture on the basis of specified interaction criteria. The method has the advantage of screening th e entire proteome simultaneously, unlike two-hybrid systems or phage display methods which can only detect proteins binding to a single bait protein at a time. The method is applicable to the study of protein-protein interactions in biopsy and autopsy specimens, to the study of protein-protein interactions i n the presence of signalling molecules, pharmacological agents or toxins, and for comparison of diseased and normal tissues or cancerous and untransformed cells. |
priorityDate | 2002-08-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 286.