Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c7ae609b1f14b3fb56a927f7b5c15b67 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H1-08 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H1-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate |
2003-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f5b82921c23aa4d98f2aa31177bb669e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_232dd63ba98f18888d4954a1fb0f9e98 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b8604a9420dc4f78f60209f0937bd030 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9fdfd6d85f8ad9244d24cff778c1bcc7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e6028de18afdaeca7223f24531b9de08 |
publicationDate |
2004-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CA-2488616-A1 |
titleOfInvention |
Isolation of nucleic acids using a polycationic polymer as precipitation agent |
abstract |
The present invention relates to a method of isolating a nucleic acid from a biological solution, which method comprises to selectively precipitate the desired nucleic acid by adding a polycationic precipitating agent to the solution and allowing it to form a complex with said nucleic acid, wherein t he precipitating agent is a highly charged linear polymer that comprises quaternary amino groups. The polycationic precipitating agent is preferably added in such an amount that the charge ratio [+] / [-] between polycationic precipitating agent and nucleic acid is >= 0.5, preferably >= 0.9 and most preferably >=1 during the precipitation, and in the presence of a salt concentration ensuring the quantitative specific precipitation of the nuclei c acid/polycation complex. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-9347055-B2 |
priorityDate |
2002-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |