http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2487538-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_db5c96f74bd5f3f369672c3a3f8be253 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2003-05-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_de3761d2ad1c7010d642313c131c976d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0de92648b8b98af838ebb1a4c3025a93 |
| publicationDate | 2003-12-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2487538-A1 |
| titleOfInvention | Amplification of ribonucleic acids |
| abstract | The invention relates to methods for the amplification of ri-bonucleic acids, comprising the following steps: (a) a single stranded DNA is produced from an RNA by means of reverse tran-scription, using a single-stranded primer having a defined se-quence, an RNA-dependent DNA polymerase and deoxyribonucleo-side triphosphates; (b) the template RNA is removed; (c) a DNA duplex is produced by means of a single-stranded primer com-prising a box sequence, a DNA polymerase and deoxyribonucleo-side triphosphates; (d) the duplex is separated into single--stranded DNAs; (e) DNA duplexes are produced from one of the single-stranded DNAs obtained in step (d) by means of a sin-gle-stranded primer comprising a promoter sequence at its 5'end and the same defined sequence as the primer used in step (a) at its 3'end, a DNA polymerase and deoxyribonucleoside triphosphates; (f) a plurality of RNA single strands, both ends of which comprise defined sequences, are produced by means of an RNA polymerase and ribonucleoside triphosphates. The invention also relates to kits for amplifying ribonucleic acids according to one of said methods, said kits comprising the following components: (a) at least at least one single--stranded primer, which contains a promoter sequence; (b) at least one single-stranded primer comprising a box sequence; (c) an RNA-dependent DNA polymerase; (d) deoxyribonucleoside triphosphates; (e) a DNA-dependent DNA polymerase; (f) an RNA polymerase; and (g) ribonucleoside triphosphates. |
| priorityDate | 2002-05-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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