| abstract |
The invention provides an isolated or purified nucleic acid molecule consisting essentially of a nucleotide sequence of at least 1877 contiguous nucleotides of (a) SEQ ID NO: 1 or (b) a nucleotide sequence encoding an angiogenic factor, wherein the nucleotide sequence comprises cDNA comprising exons 1-5 of a vascular endothelial growth factor (VEGF) joined to genomic D NA comprising introns 5, 6, and 7 and exons 6, 7, and 8 of VEGF and further comprising a mutation in one or more of the splice donor, branch point, and splice acceptor regions contained in an exon selected from the group consisting of exons 5, 6, 7, and 8, wherein the mutation promotes the production of one isoform of VEGF as compared to another isoform of VEGF. Expression constructs, compositions, and cells comprising such a nucleic aci d molecule also are provided by the invention. |