| abstract |
The invention relates to a method for enriching a population of cells. keratinocyte strains, from a preparation of keratinocytes, including a step of pre-enrichment in stem cells keratinocytic by adhesion of said preparation to a component of extracellular matrices, especially recognized by molecules involved in adhesion of keratinocytes, in particular by receptors of the family of integrins, in particular collagen, for a period of approximately 5 min to approximately 30 mins, especially around 10 mins to around 20 mins, preferably 12 mins, and the recovery of stem cells having adhered to the above component of extracellular matrices by detachment under conditions allowing maintain the viability of adhered stem cells, in particular by trypsination, in particular using 0.05% trypsin and 0.02% EDTA, for obtain a population of adherent cells. |