| abstract |
A method to improve neural cell viability in brain or spinal cord tissue aft er brain or spinal cord injury or surgery is provided. This method comprises applying a sterile liquid medium to the brain or spinal cord tissue, wherein the sterile aqueous liquid medium comprises 0 to about 3000 ~M CaCI2, about 0.1 to about 1.2 ~M Fe(NO3)3, about 2500 to about 10000 ~M KCI, 0 to about 4000 ~M MgCI2, about 30000 to about 150000 ~M NaCI, about 100 to about 30000 ~M NaHCO3, about 250 to about 4000 ~M NaH2PO4, about 0.01 to about 0.4 ~M sodium selenite, about 0.2 to about 2 ~M ZnSO4, about 2500 to about 50000 ~M D- glucose, about 1 to about 50 ~M L-carnitine, about 3 to about 80 ~M ethanolamine, about 15 to about 400 ~M D(+)-galactose, about 40 to about 800 ~M putrescine, about 20 to about 500 ~M sodium pyruvate, and growth-promotin g essential fatty acids, hormones, amino acids, vitamins and anti-oxidants in amounts effective for neuron growth, and wherein the medium is essentially free of ferrous sulfate, glutamate, and aspartate. |