| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_966a6c1f382ec9bcb5e3839dfd73e5b8 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0034
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
2002-06-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0f892731bc108062ad5d061038aa4d2e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_414e1082674285f7cf0ff2a344f228e1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec69edb2c14c6a6a3e8802699a62cecb
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9026ee9acfd2e1727a5e310dd138a626
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_395be29d022e20a6672070bc556b92ee |
| publicationDate |
2003-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CA-2453864-A1 |
| titleOfInvention |
A walk-through technique for in vitro recombination of polynucleotide sequences |
| abstract |
A simple and efficient method for random in vitro mutagenesis and recombination of polynucleotide sequences based on walk-through with chain- elongating molecules and chain terminating molecules e.g. dNTPs/ddNTPs followed by reassembly and amplification is described. The utility of this method for improving protein structure and/or function is demonstrated by creating novel sarcosine oxidase variants and by recombining human placental alkaline phosphates and calf intestinal alkaline phosphatase for better mutants with improved properties. |
| priorityDate |
2001-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |