http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2453189-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c27abc8e88132c40fa3b1779b1149d10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6897 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6897 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2002-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d1bd4f238cb8b269d7c6a4d52c22144 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7d44f22ab0ecf3beeec3185ae36c4bde http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_84bdb696552cf6ed9a83f27e7f296206 |
| publicationDate | 2003-01-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2453189-A1 |
| titleOfInvention | Bioassay for myostatin |
| abstract | The present invention relates to a bioassay for determining the amount of active myostatin present in an animal or part thereof comprising of steps (a) amplifying the myostatin promoter, or a functional fragment or variant thereof; (b) cloning the promoter into a reporter vector or construct; (c) transfecting the vector into a myogenic precursor cell line (myoblasts) and selecting transfected myoblasts; (d) incubating transfected myoblasts with myostatin, serum or muscle extracts taken from animal of interest; (e) washing the myoblasts and preparing protein extracts therefrom; (h) performing a reporter gene assay on the protein extracts. |
| priorityDate | 2001-07-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 75.