| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6ebe8446843d92527d56c6acbccba399 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K48-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K38-00 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-87
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K47-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-87
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K38-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K47-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N13-00 |
| filingDate |
2002-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3eae96b9c18c7882d9217d280586d78a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db452894d5165b674a0bf75b4d3d30a4
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7eed57811a27bd2501abe5a648b0a878
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7099b79b041e476c1f4e46313936fd6c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4ea49865088ecb8762af4f525b1b473a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9d800b176c3a06ba4aa156c15eed524d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_848926ce74cebcb8f743fa04f214db76
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_79b49f9d564935e85903a4da45eb9483
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ee330d3085189a66c61da7553df4a854
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1517fd323de066f1e7063b2ebb185137
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5e74031609f2d6de227f0da370f5acbd |
| publicationDate |
2002-10-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CA-2445260-A1 |
| titleOfInvention |
Buffer solution for electroporation and a method comprising the use of the same |
| abstract |
The invention relates to a buffer solution for suspending animal or human cells and for dissolving biologically active molecules in order to introduce said biologically active molecules into the cells using an electric current and to a method for introducing biologically active molecules into animal or human cells using an electric current and a buffer solution. The inventive buffer solution has a buffering capacity of at least 20 mmol *l-1*pH-1 and a n ionic strength of at least 200 mmol *l-1 during a change to the pH value fro m pH 7 to pH 8 and at a temperature of 25 ~C. The use of a buffer solution of this type in the corresponding method allows biologically active molecules t o be introduced into animal and human cells with a high degree of transfection efficiency and at the same time a low cell mortality. Different cell types, in particular dormant and actively dividing cells of low activity, can be successfully transfected in said buffer solution. |
| priorityDate |
2001-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |