http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2444480-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_076e36dad0616dc807a09515ce604090 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4716 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-113 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-113 |
| filingDate | 2002-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_730e86dc788b905a119067b37a40c8cf |
| publicationDate | 2002-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2444480-A1 |
| titleOfInvention | Methods and compositions for production of recombinant peptides |
| abstract | This invention entails a method for solubilizing and recovering, in bioactiv e and isolated form with retained native state configuration, a target peptide fro m a host organism in which the heterologous polypeptide is present in insoluble form. Broadly this method comprises (i) disrupting the host cell to produce a lysa te (ii) recovering lysate precipitate containing the polypeptide (iii) resuspending the lysate precipitate in a denaturant-free, nan-buffered solubilization solutio n to produce a solubilization preparation that comprises both sodium hydroxide between about 8 and about 10 mM and the target peptide between about 1 and about 4 mg peptide per ml solubilization solution, wherein the resultant solubilization preparation has a pH of between about 9 and about 11.2; (iv) recovering supernatant from the solubilization preparation containing non- denatured target peptide. Optionally, stabilizing compounds and detergents a re employed. The invention further comprises isolated insoluble proteins in bioactive form and native state configuration. |
| priorityDate | 2001-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 581.