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filingDate 2001-12-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2011-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f6af67935b918849ccd62e32cfbcf237
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publicationDate 2011-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CA-2431170-C
titleOfInvention Method of analyzing expression of gene
abstract A cDNA is prepared from mRNA. The prepared cDNA is subjected to incision with two different types of restriction enzymes. The sequence of a portion of each of the fragments obtained as a result of treatment is determined, and the fragments are classified on the basis of the determined sequence by using a primer set. At the same time, the fragments are amplified in a manner that the relative expression magnitude thereof continues to be reflected therein. When the fragments have been classified into a predetermined number of fractions, the respective fractions are subjected to electrophoresis, and a gene expression profile is produced from the results.
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