http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2424435-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_bb90ef766897ef303a351786c4d9960a http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f84747e8d08b1023763356d0e7d1db68 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/H10K85-761 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y10-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6825 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/H01L51-30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/H01L51-00 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B82B3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B82B1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-566 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-551 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-483 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N37-00 |
| filingDate | 2001-09-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7aa7ef5c4a2301a2befbe0e0fca1c77f |
| publicationDate | 2002-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2424435-A1 |
| titleOfInvention | Dendritically amplified detection method |
| abstract | A method and system for the detection of a target nucleic acid in a sample solution. The target nucleic acid comprises a first and a second end sequenc e, one of the end sequences being a 5' end sequence and the other end sequence being a 3' end sequence. The method comprises: (a) attaching to a solid surface a first oligonucleotide probe, at least a portion of which is complementary to the first end sequence of the target nucleic acid; (b) contacting the solid surface with the sample solution, thereby allowing the first probe to bind the target nucleic acid; (c) providing a second semiconductor nanoparticle to which has been attached a second oligonucleoti de probe, at least a portion of which is complementary to the second end sequen ce of the target nucleic acid; (d) contacting the solid surface of step (b) wit h the second nanoparticle, thereby allowing the second probe to bind the bound target nucleic acid; (e) providing a first semiconductor nanoparticle to whi ch has been attached the first oligonucleotide probe and pre-incubating the fir st nanoparticle with the target nucleic acid, thereby allowing the first probe to bind the target nucleic acid; (f) contacting the solid surface of step (d) with the pre-incubated first nanoparticle, thereby allowing the target nucle ic acid bound to thefirst probe to bind the second probe on the second nanoparticle; and (g) detecting the presence of the nanoparticles on the sol id surface, thereby detecting the target nucleic acid. |
| priorityDate | 2000-10-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.