http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2407703-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0c4322cb4de342be2dbedcbe0c278f6f |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2458-15 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6803 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6851 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 |
| filingDate | 2001-04-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5bc34ac37d100c949ce3d78d0f063e88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_27da31bc455db5982ed559da1428b50e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2df8fb6b2b357537d7dfc0b1cb9d48f7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ff802e183d7361d2fd92810859e1bc0b |
| publicationDate | 2001-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2407703-A1 |
| titleOfInvention | A quantitative, high-throughput screening method for protein stability |
| abstract | In proteomic research, it is often necessary to screen a large number of polypeptides for the presence of stable structure. Described, herein, are methods (referred to as MALDI MS-HX and SUPREX) for measuring the stability of proteins in a rapid high-throughput fashion. The method employs hydrogen exchange to estimate the stability of quantities of unpurified protein extracts, using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. A method of quantitatively determining the stability of a test protein under native conditions is disclosed. The method includes the steps of (a) providing a test protein; (b) contacting the protein with an exchange buffer comprising a denaturant and deuterium, the exchange buffer having a denaturant concentration; (c) contacting the protein with a mass spectrometr y matrix medium; (d) determining a change in mass of the test protein by mass spectrometry; (e) varying the denaturant concentration of the exchange buffe r; (f) repeating steps (a)-(e) a desired number of times; and (g) quantitativel y determining protein stability based on the change in mass of the test protei n as a function of denaturant concentration, whereby the stability of a test protein under the native conditions is quantitatively determined. |
| priorityDate | 2000-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 287.