abstract |
To provide an immortalized capillary pericyte line which maintains the original function/property of the cell line-deriving tissue, its establishme nt method, and the screening method for useful substance using the immortalized capillary pericyte line. Cerebral tissue of a transgenic rat carrying the large T antigen gene of SV40 thermo-sensitive mutant line tsA58 is homogenized and the resultant brain capillaries are treated with protease, thus obtained brain capillary cells are subcultured to establish a n immortalized cell that expresses SV40 thermo-sensitive large T antigen, PDGF receptor a , and Angiopoietin-1. In addition, the immortalized vascular pericyte line has ability to deposit calcium on matrix by dense culture. |