| abstract |
The present invention relates to the field of bicyclic DNA analogues, e.g. L NA and LNA modifications, which are useful for designing oligomers that form hi gh affinity duplexes with complementary RNA wherein said duplexes are substrate s for RNase H. The oligonucleotides may be partially or fully composed of LNA analogues with very high affinity and ability to recruit RNase H. The implications are that oxy-LNA by itself may be used to construct novel antisense molecules with enhanced biological activity. Alternatively, oxy-LN A may be used in combination with non-oxy-LNA, such as standard DNA, RNA or other analogues, e.g. thio-LNA or amino-LNA, to create high affinity, RNase H recruiting anti-sense compounds without the need to adhere to any fixed desi gn. |