http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2384528-C
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dea97595058f316a875c28a64efb90da |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-523 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L3-52 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01L7-52 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L7-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01L3-00 |
| filingDate | 2000-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2010-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d490363464f55e43e093efe37e88ec7e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b4248a6e7d68da7f550e2522c9fc650e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6178edfcfd43af70aa562f4e6df9b4fe http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8248ff6728504c54b2faf3986d076156 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b4ba7e54c7e3315f2052e74c76b24417 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_85a09bc74168e2e4fe79b7d7fd7988fb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_94f48f28c33a612ff91141e4e0c36fd8 |
| publicationDate | 2010-07-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2384528-C |
| titleOfInvention | Reaction system for performing in the amplification of nucleic acids |
| abstract | A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions. |
| priorityDate | 1999-09-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.