Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d202e9e866984fe229f6facc38f1d740 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P33-10 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-465 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K31-7048 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-15 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P33-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-52 |
filingDate |
2000-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52aef903ee223585953018d23b3ffd00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b88061622d8a0c47172a9c2280843c8f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7329a092bf6f18b5be794b4035f56e71 |
publicationDate |
2001-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CA-2380639-A1 |
titleOfInvention |
Cloning of the <i>streptomyces avermitilis</i> genes for glycosylation of avermectin aglycones |
abstract |
A cluster of genes involved in the synthesis and/or addition of oleandrose t o avermectin aglycones has been cloned. A 11-kb PstI clone complemented 28 avermectin glycosylation mutants in seven complementation classes. Sequencing of an 10-kb region identified 9 ORFs and an additional partial OR F. Eight of the ORFs were correlated to the seven glycosylation complementation classes. Sequence comparison to Genbank databases identified 6 genes: dTDP- glucose synthase; dTDP-glucose 4,6 dehydrase; dTDP-4-keto-hexose reductase; dTDP-hexose 3,5 epimerase; dTDP-hexose 3' O-methylase; and an avermectin aglycone-dTDP-oleandrose glycosyltransferase. The ninth ORF was essential fo r biosynthesis of the avermectin aglycones. The partial ORF encoded part of an avermectin polyketide synthase module 7. |
priorityDate |
1999-07-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |