| abstract |
The present invention provides a method for replicating virus to high titer in cultured mammalian cells by infecting the mammalian cells with the high tite r virus strain to obtain infected cells, specifically, attenuated dengue virus strains of serotype 1, 2, 3, and 4. The resulting replicated virus is suitab le for use in vaccines and vaccination methods which are also provided by the invention. |