http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2364579-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d6c9201213e4a966d85cbcd61f66e805 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-20 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 |
| filingDate | 2000-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e93c727c7cce914f48bca57827a8a871 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1168ee22c4feada60e1e18ee3caf6576 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_43c3358952d7d3bb50e459c9253dfb76 |
| publicationDate | 2000-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2364579-A1 |
| titleOfInvention | Culture of mycobacteria |
| abstract | The present invention provides a method of culture of mycobacteria which comprises culturing the mycobacteria, either in batch or continuous culture, with agitation and in the presence of sufficient detergent so that a substantially homogenous suspension of mycobacterial cells is maintained. According to a preferred method the mycobacteria are grown in continuous culture, at a temperature of 35 ~C +/- 10 ~C, at a dissolved oxygen tension of at least 1 percent, at a pH of 6.9 +/- 0.9, at a dilution rate of at least 0.02 h-1 and with agitation in the presence of sufficient detergent to maintain a substantially homogenous suspension of single cells. The present invention also provides a growth medium for culture of mycobacteria, comprising a carbon source, a mitogen, trace elements comprising at least Mg, K, P and S, and a nitrogen source. According to a separate aspect of the invention, there is provided a method of culture of a mycobacteriophage, comprising culture of mycobacteria as described above, and contacting said mycobacteria with a mycobacteriophage. |
| priorityDate | 1999-03-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 86.