| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e4feda2a82a4adcd92a7619df2248333 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
1999-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3ff53738b3ad1d0092e4ffc65dc0d7b3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ae92870e588973fed42fd15e14b26a87
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_361f16f680ed0e7344790c0351db5db1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_531c7467e11fe7bd15a7829bcd53654f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bfcb0a300157ee3157eac5fa38f6f17e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_16e0108b003a750a7b632b7dd5a03994
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dfca8ecf3fd400c820d53b8b0c724b91
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_310ae3dd4d888ce043ced16a8117b7d3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_603c0609c03a3675f1d01df2b408d4e3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cceaef4bf9dac0dbf7028c3236a16421
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69ed9defd5c3c0c27d3031e25319201f |
| publicationDate |
2000-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CA-2342393-A1 |
| titleOfInvention |
A rapid pcr method for synthesizing dna |
| abstract |
A method for synthesizing a DNA whereby the time required in the DNA synthesis by the polymerase chain reaction (PCR) method can be shortened, characterized in that PCR is effected under the conditions (A) and (B) as will be defined below with the use of DNA polymerase in an amount effective in giving more than 10 ng of amplified DNA fragments of about 2 kb per 50 µl of a liquid reaction mixture: (A) using 50 µl of a liquid reaction mixture containing DNA polymerase, 1 ng of Escherichia coli genomic DNA and 10 pmol portions of primers Eco-1 and Eco-2 having the base sequences represented respectively by SEQ ID NOS: 1 and 2 in Sequence Listing, and having a composition adequate for the DNA polymerase; and (B) effecting the PCR for 35 cycles with each cycle consisting of 1 second at 99 ~C and 7 seconds at 66 ~C; a DNA synthesis kit to be used in this DNA synthesis method; and a PCR reagent product. The above method makes it possible to quicken operations in genetic engineering studies and the genetic engineering industry. |
| priorityDate |
1998-09-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |