| abstract |
A method is provided for producing motif-specific, context-independent antibodies which are specific to at least one fixed amino acid residue whether modified or unmodified, in the context of variable surrounding amino acid or peptide sequences. The method includes the steps of (1) constructing a peptide library featuring at least one fixed amino acid and variable surrounding amino acids, and (2) immunizing a host with this peptide library. Antibodies may optionally be isolated and purified from the antisera of this immunized host. The disclosed method encompasses both modified motifs, such as phosphothreonine, phosphoserine, MAPK consensus recognition sites, 14-3-3 consensus recognition sites, CDK consensus recognition sites, and acetylated-lysine, as well as unmodified motifs. |