http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2332518-C
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_84d9adaa7e7ce01ce8020f5c721aba10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 1999-05-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2009-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9e74edbac5a7c6fe7a34a2d934b20d5e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_16da91a24d19f3bca93e0c4b8076328a |
| publicationDate | 2009-07-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2332518-C |
| titleOfInvention | Method for using unequal primer concentrations for generating nucleic acid amplification products |
| abstract | The method provided herein is a method for detecting a target sequence in a test sample. Generally, the method comprises forming a reaction mixture comprising a test sample, amplification reagents, a first primer, and a second primer wherein the concentration of the first primer in the reaction mixture is 15 % to 250 % greater than the concentration of the second primer. The target sequence is amplified according to any amplification protocol that employs the primer sequences to generate copies of the target sequence that include a product from the first and second primers. A probe is hybridized to the amplificatio n product from the first primer to form a hybrid complex; and the hybrid complex is detected as an indication of the presence of the targe t sequence in the test sample. |
| priorityDate | 1998-05-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 84.