| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dba21d4cf11e8f7ea943fa8123caf413 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-10351
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-10322
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-8813
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-345
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2030-625
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-075 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N30-96
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-363
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01J41-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-00 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-62 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-48
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-483
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J20-285
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-96
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-075
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01J41-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-36 |
| filingDate |
1999-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate |
2010-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_22b91572dcf8e1644f2193db592d8580
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f469aeee1f788dfcae276b2b2068047
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d86e24c5a591f2eae9e734858ec584c0 |
| publicationDate |
2010-11-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CA-2328462-C |
| titleOfInvention |
Efficient purification of adenovirus |
| abstract |
A method of enriching a solution of an adenovirus comprising applying a mixed solution comprising an adenovirus and at least one undesired type of biomolecule to an anion exchange chromatography resin containing a binding moiety selected from the group consisting of dimethylaminopropyl, dimethylaminobutyl, dimethylaminoisobutyl, and dimethylaminopentyl and eluting the adenovirus from the chromatography resin. Also provided is a method of purifying an adenovirus from adenovirus-infected cells comprising lysing such cells, applying the lysate to a single chromatography resin, eluting the adenovirus from the chromatography resin, and collecting a fraction containing adenovirus that is substantially as pure as triple CsC1 density gradient purified adenovirus. The present method further provides a method of accurately quantifying the number of adenoviral particles in a solution of adenovirus comprising applying to and eluting from an anion exchange chromatography resin a sample solution of adenovirus, comparing the absorbance of the sample solution of adenovirus and the absorbance of a standard solution of adenovirus, and quantifying the number of adenoviral particles in the sample solution. |
| priorityDate |
1998-04-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |