http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2321119-A1
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_eb9c6b40161bafe7bd55f0b4defcea04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_230f1799c4f7346bf5636de08fc786e2 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6837 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D311-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D311-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D495-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N37-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 |
| filingDate | 1999-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_59ee731e8eff50edfb3ec3d53067fbca |
| publicationDate | 1999-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2321119-A1 |
| titleOfInvention | Method for identifying mismatch repair glycosylase reactive sites, compound and uses thereof |
| abstract | The present application discloses a method of identifying mutations in a target DNA sequence. The method involves: (a) hybridizing the target DNA sequence with a control DNA sequence wherein said control DNA sequence is the wild-type DNA sequence corresponding to the target DNA sequence to create a duplex; (b) treating the duplex to remove any spontaneous aldehydes; (c) reacting the duplex with a repair glycosylase to convert any mismatched sites in the duplex to reactive sites containing an aldehyde-containing abasic site; (d) reacting the duplex with a compound of the formula X-Z-Y, wherein X is a detectable moiety, Y is NHNH2, O-NH2 or NH2, and Z is a hydrocarbon, alkylhydroxy, alkylethoxy, alkylester, alkylether, alkylamide or alkylamine, wherein Z may be substituted or unsubstituted; and wherein Z may contain a cleavable group; for a sufficient time and under conditions to covalently bind to the reactive sites; (e) detecting the bound compound to identify sites of mismatches; (f) determining where the mismatch occurs; and (g) determining whether the mismatch is a mutation or polymorphisms. |
| priorityDate | 1998-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 427.