http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CA-2319662-C
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7cce123535acd084e7fff1eef26ca839 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-143333 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 1999-01-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2009-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a58509ad2b9338546f3b98b3887d3cac http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc8110a9136a70b2d38481441d2de2af http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_df4d70f0054b8023a33080a30cd67b43 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aa9168809f4f0bc085084640e1e364f1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fcc008fca6d43cb21385fa0823fc681c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_26a66e7be87c97f974269cc2d7cfdef8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea64a8c6a4eb50c3feb759781363615c |
| publicationDate | 2009-11-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CA-2319662-C |
| titleOfInvention | Modified nucleic acid probes and uses thereof |
| abstract | Disclosed is a method of detecting a nucleic acid sequence of interest in a sample, the method comprising contacting the sample with first and second probes; the first probe comprising a portion complementary to the sequence of interest and so capable of hybridising thereto, and a portion non-complementary to the sequence of interest; the second probe comprising a portion complementary to the sequence of interest and so capable of hybridising thereto, and a portion non-complementary to the sequence of interest but complementary to that portion of the first probe which is non-complementary to the sequence of interest, such that the first and second probes are capable of hybridising to the sequence of interest in an adjacent or substantially adjacent manner, so as to allow complementary portions of the first and second probes to hybridize to each other; causing extension of the first probe with a nucleic acid polymerase, using the second probe as a template; and detecting directly or indirectly the extension of the first probe, so as to indicate the presence of the sequence of interest; characterised in that the first and/or second probe comprises a destabilizing moiety which cannot base pair with the reciprocal probe, thereby preventing hybridisation of the first and second probes in the absence of the sequence of interest. |
| priorityDate | 1998-01-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 178.