| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5181f7bc41146d952cf4e72f866a39ca |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A40-146 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8237
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8239
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N65-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-8285
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N65-00 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N65-08
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-29
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate |
1998-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c9471fb4605051e149ccc105dac87ea
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_567d1054839c75cd3d265ee6b379ec45
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c4561a693458d0056b14739539cc89e7 |
| publicationDate |
1999-06-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CA-2311571-A1 |
| titleOfInvention |
Isolation and characterization of plant regulatory sequences |
| abstract |
In the search for plant regulatory sequences capable of driving nematode-triggered effector gene expression in feeding structures the promoter tagging appears to be a valuable tool. A large collection of transgenic Arabidopsis thaliana(L.) plants was generated. They were transformed with a .beta.-glucuronidase gene functioning as a promoter tag. Two T-DNA constructs, pGV1047 and p.DELTA.gusBin19 were used. Early responses to nematode invasion were of primary interest. Four lines exhibiting GUS activity in syncytia induced by the beet cyst nematode were studied. Reporter gene activation was also identified in galls induced by root knot and ectoparasitic nematodes. Time course studies revealed that the four tags were differentially activated during the development of the feeding structure. T-DNA-flanking regions responsible for the observed responses after nematode infection were isolated and characterized for promoter activity. |
| priorityDate |
1997-11-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |