| abstract |
The invention relates to a process for the production of a stable compound (+) catechin penta acetate of formula (2) from T. wallichiana tissue cultures and production of compound (+) catechin of formula (1), said process comprising the steps of inoculation of explants on culture media supplemented with auxins and cytokinins, incubation of the cultures for callus initiation followed by subculturing at 4-6 weeks intervals, extraction of fresh pulverized calli with polar solvents, evaporating the polar solvent to give a residue, treating t:he residue with a chlorinated solvent to isolate (+) catechin by filtration or treating the residue with water and extracting with a chlorinated solvent and evaporating the solvent to semi-solid mass, subjecting the resultant mass to column chromatography over suitable adsorbents, eluting with organic solvent or mixtures of organic solvent to obtain(+)catechin penta acetate. |